Ficin
CA89168-042
:
- Size:5 ml resin
- Enzyme name:Ficin
- Enzyme synonyms:Ficain
- Cat. no.:CA89168-042
- Supplier No.:786-793
Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge reason
Immobilized Ficin is a convenient reagent for producing Fab and F(ab')2 fragments as it avoids the need to remove the ficin enzyme after digestion.
Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1mM or 10mM cysteine, ficin generates F(ab')2 and Fab fragments respectively. The Fab and F(ab')2 fragments can be separated from whole IgG and Fc with either Immobilized Protein A or ion exchange chromatography.
: Accessories not included.