You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
Human transferrin, CF™750 Conjugate
Supplier: Biotium
Transferrin is a glycoprotein with homologous N-terminal and C-terminal iron-binding domains that binds up to two Fe(III)atoms for delivery to vertebrate cells through receptor-mediated endocytosis
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Mix-n-Stain™ CF® Dye Small Ligand Labeling Kits, Biotium
Supplier: Biotium
Easily label SNAP-tag®, CLIP-tag™, HaloTag®, or other ligands with fluorescent dyes, no purification needed. Cell membrane-permeant and impermeant dye options for intracellular or surface targets.
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CF®555 hydrazide
Supplier: Biotium
Fluorescent CF® Dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.
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Reactive CF® Dyes and Protein Labeling Kits, Biotium
Supplier: Biotium
Biotium offers a number of reactive dye formats for labelling proteins, nucleic acids, and other biomolecules with fluorescent dyes. CF® dyes offer advantages in brightness and photostability compared other similar commercial dyes. CF® dyes are available in a number of reactive dye formats for CF® dye colours span the visible and near-infrared spectra.
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Reactive Oxygen Species Indicators, Biotium
Supplier: Biotium
Commonly used fluorescent dyes for detection of oxidative activities in cells.
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Biotin-16-dUTP, Biotium
Supplier: Biotium
Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. Biotin-dUTP conjugates are available wtih 11-carbon, 16-carbon, or 20-carbon linker lengths.
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R-Phycoerythrin (RPE), Columbia Biosciences
Supplier: Columbia Biosciences
Many applications and instruments were developed specifically for R-phycoerythrin
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Biotin NTA, Biotium
Supplier: Biotium
Biotinylated probes for detection of His-tagged proteins.
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Annexin V, CF® 770 conjugate
Supplier: Biotium
Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for in vivo imaging.
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CF®488A Alkyne
Supplier: Biotium
CF® Dye alkynes react with azide groups via copper-catalyzed bioorthogonal cycloaddition. Can be used to fluorescently detect or label azide groups on target molecules. CF® Dye alkynes can also be used as building blocks to form fluorescent polymers.
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Reactive Dye Conjugates, Biotium
Supplier: Biotium
Reactive dyes containing an amine functional group.
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Biotin-11-dUTP, Biotium
Supplier: Biotium
Biotin-11-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling.
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CF®405S BCN
Supplier: Biotium
CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labeling reactions are also available.
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CF™ Dye Streptavidin and Monoclonal Mouse Anti-Biotin Conjugates, Biotium
Supplier: Biotium
Biotium offers a variety of streptavidin and anti-biotin antibody conjugates featuring our series of CF dyes
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Colorimetric and Fluorogenic Enzyme Substrates, Biotium
Supplier: Biotium
Popular chromogenic and fluorogenic substrates for phosphatases, peroxidases, and other enzymes.
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ExoBrite™ STORM CTB EV Staining Kits, Biotium
Supplier: Biotium
Fluorescent cholera toxin subunit B (CTB) conjugates that are optimized for direct stochastic optical reconstruction microscopy (dSTORM) imaging of EVs and exosomes.
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B-Phycoerythrin (BPE), Columbia Biosciences
Supplier: Columbia Biosciences
Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore
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D-Luciferin Luminescent Substrates, Biotium
Supplier: Biotium
Luciferin is a substrate for the enzyme firefly luciferase
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CF®680 Azide
Supplier: Biotium
CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.
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ReadiLink™ Cy3 Nick Translation dsDNA Labeling Kit
Supplier: AAT BIOQUEST INC
It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.
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CF™ Dyes Cholera Toxin Subunit B, CF™ Dye Conjugates, Biotium
Supplier: Biotium
Cholera toxin subunit B conjugated to Biotium's next-generation fluorescent CF™ dyes.
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CF™ Dye Lectin Conjugates, Biotium
Supplier: Biotium
A lectin labeled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.
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Phalloidin Conjugates, Biotium
Supplier: Biotium
A highly selective actin filament stain for fixed and permeabilized cells. Labeled phalloidins have similar affinity for both large and small filaments.
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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin, Biotium
Supplier: Biotium
Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.
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Allophycocyanin (Crosslinked APC), Columbia Biosciences
Supplier: Columbia Biosciences
Allophycocyanin (APC) is a commonly used label in Time-Resolved Fluorescence Resonant Energy Transfer (TR-FRET) assays for High Throughput Screening and Flow Cytometry.
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Biotin-20-dUTP, Biotium
Supplier: Biotium
Biotin-20-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. Biotin-dUTP conjugates are available wtih 11-carbon, 16-carbon, or 20-carbon linker lengths.
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HRP WGA Wheat Germ Agglutinin (WGA) Conjugates
Supplier: Biotium
A wheat germ agglutinin (WGA) lectin labeled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.
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Pierce EZ-Link® Solid Phase Biotinylation Kits, Thermo Scientific
Supplier: Thermo Scientific
This antibody labeling system uses nickel chelated agarose to temporarily immobilize antibody molecules via their histidine-rich Fc regions
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HOOK™ Sulfhydryl Reactive Biotin Reagents and Kits for Highly Efficient Protein Labeling, G-Biosciences
Supplier: G-Biosciences
The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research
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CLIP-SURFACE® 488, New England Biolabs
Supplier: New England Biolabs (NEB)
CLIP-Surface™ 488 is a photostable green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins on the surface of living cells or in vitro