You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
Streptavidin, New England Biolabs
Supplier: New England Biolabs (NEB)
A 52.8 kDa non-glycosylated protein with a very high affinity for biotin
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Pierce™ Sulfhydryl Addition Kit, Thermo Scientific
Supplier: Thermo Scientific
Introduce free sulfhydryl groups into a protein of interest to allow the modified protein to react with a variety of sulfhydryl-selective products.
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Pierce™ Biotin 3' End DNA Labeling Kit, Thermo Scientific
Supplier: Thermo Scientific
Kit has been optimized to incorporate 1-3 biotinylated ribonucleotides (biotin-N4-CTP) onto the 3' end of DNA strands where the label will be less likely to interfere with hybridization or sequence-specific binding of proteins.
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SNAP-SURFACE® Alexa Fluor 488 , New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-Surface® Alexa Fluor® 488 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells
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Pierce™ GlycoLink™ Coupling Catalyst, Thermo Scientific
Supplier: Thermo Scientific
An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.
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Pierce™ Immunostain Enhancer, Thermo Scientific
Supplier: Thermo Scientific
The Pierce™ Immunostain Enhancer is compatible with fluorescent and chromogenic detection and routinely increases both signal intensity and detection sensitivity.
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Pierce™ NHS-Fluorescein Antibody Labeling Kit, Thermo Scientific
Supplier: Thermo Scientific
Pierce™ NHS-Fluorescein Antibody Labeling Kit is a multi-component kit containing a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.
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Pierce EZ-Link® Carboxyl Reactive Biotinylation Reagents, Thermo Scientific
Supplier: Thermo Scientific
Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatized biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4.5 - 5 and requires just minutes to complete.
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SNAP-CELL® TMR-STAR , New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-Cell® TMR-Star is a red fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells, on cell surfaces, or in vitro
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Pierce™ CA(PEG)n Carboxy-PEG-Amine Compound, Thermo Scientific
Supplier: Thermo Scientific
The carboxy-PEGn-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers
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CLIP-Biotin, New England Biolabs
Supplier: New England Biolabs (NEB)
CLIP-Biotin is a cell-permeable substrate (BC-Biotin) based on biotin with an amidocaproyl linker
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CLIP-CELL® 505 , New England Biolabs
Supplier: New England Biolabs (NEB)
CLIP-Cell™ 505 is a green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells, on cell surfaces or in vitro
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Carbohydrate Coupling Resin, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Carbohydrate Coupling Resin is designed for the simple and efficient coupling of glycoproteins to a solid agarose support through oxidized sugar groups
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Pierce™ DyLight™ PEGylated Dyes, Antibody Labelling Dyes, Thermo Scientific
Supplier: Thermo Scientific
DyLight™ PEGylated dyes display absorption spectra ranging from 550 nm to 783 nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor® or Cy® dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight™ reagents allows a high dye-to-protein ratio without precipitation during conjugation.
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Pierce™ Pegylation Reagents, Methyl-PEG-Amine Compounds, Thermo Scientific
Supplier: Thermo Scientific
The methyl-PEGn-amine (MA[PEG]n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers
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Template Switching RT Enzyme Mix, New England Biolabs
Supplier: New England Biolabs (NEB)
The reverse transcriptase (RT) in the Template Switching RT Enzyme Mix adds a few non-templated nucleotides after it reaches the 5' end of the RNA template.
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Pierce™ ABC Peroxidase Standard Staining Kit, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.
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Pierce™ PEGylated SPDP Crosslinkers, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific Pierce™ PEGn-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a n-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond.
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DyLight™ Antibody Labeling, Long Stokes Shift Dyes, Thermo Scientific
Supplier: Thermo Scientific
The DyLight™ Long Stokes Shift Dyes are amine-reactive fluorescent dyes that produce long Stokes (LS) shifts of 80 - 145 nm in excitation/emission wavelengths.
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Pierce™ PEGylated BS (bis(sulfosuccinimidyl)suberate) Crosslinkers, Thermo Scientific
Supplier: Thermo Scientific
Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.
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femtoCHROMO™- HRP Kit for Chromogenic Detection, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' femtoCHROMO™-HRP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining
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HOOK™ Activated Agarose (Amine Reactive) for Affinity Column Generation, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups