You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
Biotin NTA, Biotium
Supplier: Biotium
Biotinylated probes for detection of His-tagged proteins.
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Reactive Oxygen Species Indicators, Biotium
Supplier: Biotium
Commonly used fluorescent dyes for detection of oxidative activities in cells.
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Biotin-16-dUTP, Biotium
Supplier: Biotium
Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. Biotin-dUTP conjugates are available wtih 11-carbon, 16-carbon, or 20-carbon linker lengths.
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Annexin V, CF® 770 conjugate
Supplier: Biotium
Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for in vivo imaging.
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R-Phycoerythrin (RPE), Columbia Biosciences
Supplier: Columbia Biosciences
Many applications and instruments were developed specifically for R-phycoerythrin
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CF™ Dye Streptavidin and Monoclonal Mouse Anti-Biotin Conjugates, Biotium
Supplier: Biotium
Biotium offers a variety of streptavidin and anti-biotin antibody conjugates featuring our series of CF dyes
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B-Phycoerythrin (BPE), Columbia Biosciences
Supplier: Columbia Biosciences
Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore
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Colorimetric and Fluorogenic Enzyme Substrates, Biotium
Supplier: Biotium
Popular chromogenic and fluorogenic substrates for phosphatases, peroxidases, and other enzymes.
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Biotin-11-dUTP, Biotium
Supplier: Biotium
Biotin-11-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling.
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CF™ Dyes Cholera Toxin Subunit B, CF™ Dye Conjugates, Biotium
Supplier: Biotium
Cholera toxin subunit B conjugated to Biotium's next-generation fluorescent CF™ dyes.
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CF®405S BCN
Supplier: Biotium
CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labeling reactions are also available.
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ReadiLink™ Cy3 Nick Translation dsDNA Labeling Kit
Supplier: AAT BIOQUEST INC
It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.
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Allophycocyanin (Crosslinked APC), Columbia Biosciences
Supplier: Columbia Biosciences
Allophycocyanin (APC) is a commonly used label in Time-Resolved Fluorescence Resonant Energy Transfer (TR-FRET) assays for High Throughput Screening and Flow Cytometry.
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ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit
Supplier: AAT BIOQUEST INC
It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.
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Thiol-Reactive Fluorescent Dyes and Other Thiol-Modifying Reagents, Biotium
Supplier: Biotium
MTS, TS, and maleimide, and other thiol-modifying reagents and reactive fluorescent dyes.
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ExoBrite™ STORM CTB EV Staining Kits, Biotium
Supplier: Biotium
Fluorescent cholera toxin subunit B (CTB) conjugates that are optimized for direct stochastic optical reconstruction microscopy (dSTORM) imaging of EVs and exosomes.
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D-Luciferin Luminescent Substrates, Biotium
Supplier: Biotium
Luciferin is a substrate for the enzyme firefly luciferase
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VivoBrite™ rapid antibody labeling kit for small animal in vivo imaging, CF™790 SE
Supplier: Biotium
Near-infrared dyes offer highly sensitive and specific detection in complex biological systems, because cells and tissues have intrinsically low autofluorescence in the near-IR region
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HRP WGA Wheat Germ Agglutinin (WGA) Conjugates
Supplier: Biotium
A wheat germ agglutinin (WGA) lectin labeled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.
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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin, Biotium
Supplier: Biotium
Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.
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Mix-n-Stain™ CF® Dye Small Ligand Labeling Kits, Biotium
Supplier: Biotium
Easily label SNAP-tag®, CLIP-tag™, HaloTag®, or other ligands with fluorescent dyes, no purification needed. Cell membrane-permeant and impermeant dye options for intracellular or surface targets.
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Reactive CF® Dyes and Protein Labeling Kits, Biotium
Supplier: Biotium
Biotium offers a number of reactive dye formats for labelling proteins, nucleic acids, and other biomolecules with fluorescent dyes. CF® dyes offer advantages in brightness and photostability compared other similar commercial dyes. CF® dyes are available in a number of reactive dye formats for CF® dye colours span the visible and near-infrared spectra.
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Reactive Dye Conjugates, Biotium
Supplier: Biotium
Reactive dyes containing an amine functional group.
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HOOK™ Sulfhydryl Reactive Biotin Reagents and Kits for Highly Efficient Protein Labeling, G-Biosciences
Supplier: G-Biosciences
The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research
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CLIP-SURFACE® 488, New England Biolabs
Supplier: New England Biolabs (NEB)
CLIP-Surface™ 488 is a photostable green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins on the surface of living cells or in vitro
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SNAP-SURFACE® 549 , New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-Surface™ 549 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells
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SNAP-SURFACE® Alexa Fluor 546 , New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-Surface Alexa Fluor 546 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells
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Pierce™ Dye Removal Columns, Thermo Scientific
Supplier: Thermo Scientific
Pierce™ Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions.
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EZ-Link™ Micro NHS-PEG4 Biotinylation Kit
Supplier: Thermo Scientific
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.
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CF®680 Azide
Supplier: Biotium
CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.