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202 results for Protein Linking and Labeling Systems

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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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Biotin NTA, Biotium

Biotin NTA, Biotium

Supplier: Biotium

Biotinylated probes for detection of His-tagged proteins.

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Reactive Oxygen Species Indicators, Biotium

Supplier: Biotium

Commonly used fluorescent dyes for detection of oxidative activities in cells.

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Biotin-16-dUTP, Biotium

Biotin-16-dUTP, Biotium

Supplier: Biotium

Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. Biotin-dUTP conjugates are available wtih 11-carbon, 16-carbon, or 20-carbon linker lengths.

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Annexin V, CF® 770 conjugate

Annexin V, CF® 770 conjugate

Supplier: Biotium

Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for in vivo imaging.

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R-Phycoerythrin (RPE), Columbia Biosciences

R-Phycoerythrin (RPE), Columbia Biosciences

Supplier: Columbia Biosciences

Many applications and instruments were developed specifically for R-phycoerythrin

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CF™ Dye Streptavidin and Monoclonal Mouse Anti-Biotin Conjugates, Biotium

Supplier: Biotium

Biotium offers a variety of streptavidin and anti-biotin antibody conjugates featuring our series of CF dyes

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B-Phycoerythrin (BPE), Columbia Biosciences

B-Phycoerythrin (BPE), Columbia Biosciences

Supplier: Columbia Biosciences

Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore

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Colorimetric and Fluorogenic Enzyme Substrates, Biotium

Supplier: Biotium

Popular chromogenic and fluorogenic substrates for phosphatases, peroxidases, and other enzymes.

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Biotin-11-dUTP, Biotium

Biotin-11-dUTP, Biotium

Supplier: Biotium

Biotin-11-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling.

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CF™ Dyes Cholera Toxin Subunit B, CF™ Dye Conjugates, Biotium

CF™ Dyes Cholera Toxin Subunit B, CF™ Dye Conjugates, Biotium

Supplier: Biotium

Cholera toxin subunit B conjugated to Biotium's next-generation fluorescent CF™ dyes.

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CF®405S BCN

CF®405S BCN

Supplier: Biotium

CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labeling reactions are also available.

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ReadiLink™ Cy3 Nick Translation dsDNA Labeling Kit

ReadiLink™ Cy3 Nick Translation dsDNA Labeling Kit

Supplier: AAT BIOQUEST INC

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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Allophycocyanin (Crosslinked APC), Columbia Biosciences

Allophycocyanin (Crosslinked APC), Columbia Biosciences

Supplier: Columbia Biosciences

Allophycocyanin (APC) is a commonly used label in Time-Resolved Fluorescence Resonant Energy Transfer (TR-FRET) assays for High Throughput Screening and Flow Cytometry.

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ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit

ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit

Supplier: AAT BIOQUEST INC

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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Thiol-Reactive Fluorescent Dyes and Other Thiol-Modifying Reagents, Biotium

Thiol-Reactive Fluorescent Dyes and Other Thiol-Modifying Reagents, Biotium

Supplier: Biotium

MTS, TS, and maleimide, and other thiol-modifying reagents and reactive fluorescent dyes.

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ExoBrite™ STORM CTB EV Staining Kits, Biotium

ExoBrite™ STORM CTB EV Staining Kits, Biotium

Supplier: Biotium

Fluorescent cholera toxin subunit B (CTB) conjugates that are optimized for direct stochastic optical reconstruction microscopy (dSTORM) imaging of EVs and exosomes.

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D-Luciferin Luminescent Substrates, Biotium

Supplier: Biotium

Luciferin is a substrate for the enzyme firefly luciferase

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VivoBrite™ rapid antibody labeling kit for small animal in vivo imaging, CF™790 SE

VivoBrite™ rapid antibody labeling kit for small animal in vivo imaging, CF™790 SE

Supplier: Biotium

Near-infrared dyes offer highly sensitive and specific detection in complex biological systems, because cells and tissues have intrinsically low autofluorescence in the near-IR region

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HRP WGA Wheat Germ Agglutinin (WGA) Conjugates

HRP WGA Wheat Germ Agglutinin (WGA) Conjugates

Supplier: Biotium

A wheat germ agglutinin (WGA) lectin labeled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.

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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin, Biotium

Coelenterazine Substrates for Renilla Luciferase and Apoaequorin, Biotium

Supplier: Biotium

Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

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Mix-n-Stain™ CF® Dye Small Ligand Labeling Kits, Biotium

Mix-n-Stain™ CF® Dye Small Ligand Labeling Kits, Biotium

Supplier: Biotium

Easily label SNAP-tag®, CLIP-tag™, HaloTag®, or other ligands with fluorescent dyes, no purification needed. Cell membrane-permeant and impermeant dye options for intracellular or surface targets.

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Reactive CF® Dyes and Protein Labeling Kits, Biotium

Reactive CF® Dyes and Protein Labeling Kits, Biotium

Supplier: Biotium

Biotium offers a number of reactive dye formats for labelling proteins, nucleic acids, and other biomolecules with fluorescent dyes. CF® dyes offer advantages in brightness and photostability compared other similar commercial dyes. CF® dyes are available in a number of reactive dye formats for CF® dye colours span the visible and near-infrared spectra.

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Reactive Dye Conjugates, Biotium

Supplier: Biotium

Reactive dyes containing an amine functional group.

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HOOK™ Sulfhydryl Reactive Biotin Reagents and Kits for Highly Efficient Protein Labeling, G-Biosciences

HOOK™ Sulfhydryl Reactive Biotin Reagents and Kits for Highly Efficient Protein Labeling, G-Biosciences

Supplier: G-Biosciences

The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research

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CLIP-SURFACE® 488, New England Biolabs

CLIP-SURFACE® 488, New England Biolabs

Supplier: New England Biolabs (NEB)

CLIP-Surface™ 488 is a photostable green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins on the surface of living cells or in vitro

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SNAP-SURFACE® 549 , New England Biolabs

SNAP-SURFACE® 549 , New England Biolabs

Supplier: New England Biolabs (NEB)

SNAP-Surface™ 549 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells

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SNAP-SURFACE® Alexa Fluor 546 , New England Biolabs

SNAP-SURFACE® Alexa Fluor 546 , New England Biolabs

Supplier: New England Biolabs (NEB)

SNAP-Surface Alexa Fluor 546 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells

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Pierce™ Dye Removal Columns, Thermo Scientific

Pierce™ Dye Removal Columns, Thermo Scientific

Supplier: Thermo Scientific

Pierce™ Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions.

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EZ-Link™ Micro NHS-PEG4 Biotinylation Kit

EZ-Link™ Micro NHS-PEG4 Biotinylation Kit

Supplier: Thermo Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

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CF®680 Azide

CF®680 Azide

Supplier: Biotium

CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.

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