About this item
This SuperMix is a 2x concentrated, ready-to-use reaction cocktail for real-time quantitative PCR (qPCR) that contains all components, except primers, probes, and templates
- Ultrapure, hot start AccuStart enzyme technology and anti-foaming technology
- Available in two versions: Universal and Low-ROX
- Maximum assay sensitivity and target precision with stringent, ultrapure, and high-yielding AccuStart II hot start Taq DNA polymerase
- Broad linear detection range with cDNA or genomic DNA templates
- Enables highly sensitive 4 target detection assays with coamplified, robust internal positive controls
- Supports efficient vortex mixing with proprietary anti-foaming technology
A key component of this supermix is AccuStart™ Taq DNA polymerase with monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® MultiPlex qPCR SuperMix delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations.
4 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
This SuperMix is a 2x concentrated, ready-to-use reaction cocktail for real-time quantitative PCR (qPCR) that contains all components, except primers, probes, and templates
- Ultrapure, hot start AccuStart enzyme technology and anti-foaming technology
- Available in two versions: Universal and Low-ROX
- Maximum assay sensitivity and target precision with stringent, ultrapure, and high-yielding AccuStart II hot start Taq DNA polymerase
- Broad linear detection range with cDNA or genomic DNA templates
- Enables highly sensitive 4 target detection assays with coamplified, robust internal positive controls
- Supports efficient vortex mixing with proprietary anti-foaming technology
A key component of this supermix is AccuStart™ Taq DNA polymerase with monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® MultiPlex qPCR SuperMix delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations.
Documents
Specifications for Product Family
Specifications
Specifications
- Catalog No:
- CA101414-204
- CA89168-786
- CA97065-230
- 97065-230
- CA97065-232
- Application:
- qPCR probe
- qPCR probe
- qPCR probe
- qPCR probe
- qPCR probe
- Multiplexing:
- Yes
- Yes
- Yes
- Yes
- Yes
Specifications
Product Family Options
Product Information
- Cycler compatibilityNo. of reactionsAvailabilityPrice
- CA101414-204PerfeCTa® MultiPlex qPCR SuperMixPerfeCTa® MultiPlex qPCR SuperMixAgilent AriaMx, BioRad CFX, QIAGEN Rotor-Gene Q, Quantabio Q, Roche LightCycler 480200× 50 µlLog in for availabilityEach$1,055.33Supplier Number: 95063-200Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.CA101414-204ApplicationqPCR probeMultiplexingYes - CA89168-786PerfeCTa® MultiPlex qPCR SuperMixPerfeCTa® MultiPlex qPCR SuperMixAgilent AriaMx, BioRad CFX, QIAGEN Rotor-Gene Q, Quantabio Q, Roche LightCycler 4801000× 50 µlLog in for availabilityEach$5,238.77Supplier Number: 95063-01KItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.CA89168-786ApplicationqPCR probeMultiplexingYes - CA97065-230PerfeCTa® MultiPlex qPCR SuperMix, Low ROX™PerfeCTa® MultiPlex qPCR SuperMix, Low ROX™ABI 7500/7500 Fast/QuantStudio™, Agilent AriaMx, Douglas Scientific IntelliQube®, Stratagene Mx200× 50 µlLog in for availabilityEach$832.41Supplier Number: 95108-200Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.CA97065-230ApplicationqPCR probeMultiplexingYes - 97065-230PerfeCTa® MultiPlex qPCR SuperMix, Low ROX™PerfeCTa® MultiPlex qPCR SuperMix, Low ROX™ABI 7500/7500 Fast/QuantStudio™, Agilent AriaMx, Douglas Scientific IntelliQube®, Stratagene Mx200× 50 µlDiscontinued-Supplier Number: 95108-200Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.97065-230ApplicationqPCR probeMultiplexingYes - CA97065-232PerfeCTa® MultiPlex qPCR SuperMix, Low ROX™PerfeCTa® MultiPlex qPCR SuperMix, Low ROX™ABI 7500/7500 Fast/QuantStudio™, Agilent AriaMx, Douglas Scientific IntelliQube®, Stratagene Mx1000× 50 µlLog in for availabilityEach$4,028.92Supplier Number: 95108-01KItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.CA97065-232ApplicationqPCR probeMultiplexingYes
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