Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/ca/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
Specifications
- Description:PicoPump with hold pressure and vacuum
- Dimensions:43×13×24 cm (17×5.25×9.5")
- Cat. no.:77665-206
- Weight:6.3 kg
Specifications
About this item
Although syringe and piston techniques are popular for microinjection in the nanoliter range, microinjection of sub-nanoliter volumes through a very small opening in a glass micropipette is often better accomplished with a controlled burst of gas pressure. Designed to simplify intracellular injection and a variety of other microinjection tasks, WPI's PicoPumps use precisely regulated pressures for securing cells and injecting them with fluid.
- Inject into a single cell with picoliter volumes
- Regulated Hold, Ejection and Vacuum Pressure
- Carefully regulated air pressures for securing cells and injecting them with fluid
- Independent vacuum regulation
- Hold pressure prevents backfilling of the pipette by capillary action
- PV830 includes vacuum pressure for filling pipettes from the tip or securing floating cells during injection
- Power: 95 to 135 V or 220 to 240 V, 50/60 Hz
Injected volumes range from picoliters to nanoliters. Separate ports supply positive and negative pressure positive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. In the time between injections, a secondary default 'Hold' pressure is applied to the pipette to prevent fluid uptake through capillary action or diffusion. The pressure pulses are typically controlled by a precision timer.
Timing, ejection pressure, holding pressure and suction are adjusted independently by control knobs and monitored with indicator gauges on the front panel. Injection pressure is controlled by a 20-turn regulator on the front panel. The built-in timing function controls the amount of time that the injection pressure is applied with millisecond resolution. Time intervals can range from 10 s down to 10 ms or less. The pressure burst can also be controlled manually or triggered by an external source.
Specifications:
Pressure input: 0 to 120 psi
Pressure output: 0.3 to 90 psi
Pressure burst timer (10-turn dial): 10 ms to 10 s in Timed mode
Regulator accuracy: 0.1% (20-turn dial)
Regulator repeatability: 0.05 psi (both hold and eject pressures)
Gauge accuracy: 3% at full scale (both hold and eject pressures)
Input connector: Quick Connect (¹/₄" OD tubing)
Output connector: Barbed (¹/₁₆" ID tubing)
Vacuum input: 0 to 30.0" Hg
Vacuum output: 0.2 to 29.9" Hg
Lowest regulated vacuum: 3" water
Regulator accuracy: 0.1% (20-turn dial)
Regulator repeatability: 0.03" Hg
Gauge accuracy: 3% at full scale
Input connector: Quick connect (¹/₄" OD tubing)
Output Connector: Barbed (¹/₁₆" ID tubing)
Control: Manual
Vent: Atmosphere
Fuse (Older models): 120 V: 0.5 A, fast, 0.25×1.25" USA; 230 V: 0.25 A, fast, 0.25×1.25" USA
Fuse (2019 models): 120 V: 0.5 A, fast, 5×20 mm; 230 V: 0.25 A, fast 5×20 mm
Input kit: 10' nylon tubing (0.25" OD, 1000 psi), one ¹/₂" female NPT adapter
Output kit: Two PicoNozzle