Mouse Recombinant TNF-A (from E. coli)

Mouse Recombinant TNF-A (from <i>E. coli</i>)

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About this item

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.

High quality
Low endotoxin
Affordable

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High quality
Low endotoxin
Affordable

Specifications for Product Family

Specifications

Catalog No:
77660-840
77660-836
77660-832
77660-838
77660-834

Protein/peptide name:
TNF-A (AF)
TNF-A (AF)
TNF-A (AF)
TNF-A (AF)
TNF-A (AF)

Protein synonyms:
Cytotoxin|DIF|Necrosin|Cachexin|TNFSF2|TNF|Cachectin
Cytotoxin|DIF|Necrosin|Cachexin|TNFSF2|TNF|Cachectin
Cytotoxin|DIF|Necrosin|Cachexin|TNFSF2|TNF|Cachectin
Cytotoxin|DIF|Necrosin|Cachexin|TNFSF2|TNF|Cachectin
Cytotoxin|DIF|Necrosin|Cachexin|TNFSF2|TNF|Cachectin

Protein/peptide type:
Recombinant
Recombinant
Recombinant
Recombinant
Recombinant

Protein Function:
Cytokine
Cytokine
Cytokine
Cytokine
Cytokine

Species:
Mouse
Mouse
Mouse
Mouse
Mouse

Source:
E. coli
E. coli
E. coli
E. coli
E. coli

Conjugation:
Unconjugated
Unconjugated
Unconjugated
Unconjugated
Unconjugated

Gene ID:
P06804
P06804
P06804
P06804
P06804

Sequence:
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Amino acid number:
157
157
157
157
157

Protease-free:
N
N
N
N
N

Animal-free:
Y
Y
Y
Y
Y

Nuclease-free:
N
N
N
N
N

Carrier-free:
Y
Y
Y
Y
Y

Endotoxin-free:
N
N
N
N
N

Endotoxin level:
Low
Low
Low
Low
Low

Endotoxin content:
Endotoxin LAL (EU/µg) ≤0.1
Endotoxin LAL (EU/µg) ≤0.1
Endotoxin LAL (EU/µg) ≤0.1
Endotoxin LAL (EU/µg) ≤0.1
Endotoxin LAL (EU/µg) ≤0.1

Biological activity:
Bioactivity Assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D, ED₅₀ ≤0.1 ng/ml, Bioactivity ≥1.0×10⁷ units/mg
Bioactivity Assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D, ED₅₀ ≤0.1 ng/ml, Bioactivity ≥1.0×10⁷ units/mg
Bioactivity Assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D, ED₅₀ ≤0.1 ng/ml, Bioactivity ≥1.0×10⁷ units/mg
Bioactivity Assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D, ED₅₀ ≤0.1 ng/ml, Bioactivity ≥1.0×10⁷ units/mg
Bioactivity Assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D, ED₅₀ ≤0.1 ng/ml, Bioactivity ≥1.0×10⁷ units/mg

Purity:
≥95%
≥95%
≥95%
≥95%
≥95%

Grade:
RUO
RUO
RUO
RUO
RUO

Formulation:
10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5

Molecular weight:
17.4 kDa
17.4 kDa
17.4 kDa
17.4 kDa
17.4 kDa

Reconstitution instructions:
Sterile water at 0.1 mg/ml
Sterile water at 0.1 mg/ml
Sterile water at 0.1 mg/ml
Sterile water at 0.1 mg/ml
Sterile water at 0.1 mg/ml

Storage conditions:
–20 °C
–20 °C
–20 °C
–20 °C
–20 °C

Shipping temperature:
RT
RT
RT
RT
RT

Tested applications:
Bioassay
Bioassay
Bioassay
Bioassay
Bioassay

Specifications

Product Family Options

Product Information

Pack type
Size
Availability
Price
77660-840
Vial
5 µg
Log in for availability
Log in for availability
Each
Each - $133.06
$133.06
Each
$133.06
Minimum order quantity is 1
Supplier Number: 200-31AF-5UG
Specifications:
More
Specifications
Cat. No.
77660-840
Protein/peptide name
TNF-A (AF)
Protein synonyms
Cytotoxin|DIF|Necrosin|Cachexin|TNFSF2|TNF|Cachectin
Protein/peptide type
Recombinant
Protein Function
Cytokine
Species
Mouse
Source
E. coli
Conjugation
Unconjugated
Gene ID
P06804
Sequence
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
Amino acid number
157
Protease-free
N
Animal-free
Y
Nuclease-free
N
Carrier-free
Y
Endotoxin-free
N
Endotoxin level
Low
Endotoxin content
Endotoxin LAL (EU/µg) ≤0.1
Biological activity
Bioactivity Assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D, ED₅₀ ≤0.1 ng/ml, Bioactivity ≥1.0×10⁷ units/mg
Purity
≥95%
Grade
RUO
Formulation
10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Molecular weight
17.4 kDa
Reconstitution instructions
Sterile water at 0.1 mg/ml
Storage conditions
–20 °C
Shipping temperature
RT
Tested applications
Bioassay
77660-836
Vial
20 µg
Log in for availability
Log in for availability
Each
Each - $336.87
$336.87
Each
$336.87
Minimum order quantity is 1
Supplier Number: 200-31AF-20UG
Specifications:
More
Specifications
Cat. No.
77660-836
Protein/peptide name
TNF-A (AF)
Protein synonyms
Cytotoxin|DIF|Necrosin|Cachexin|TNFSF2|TNF|Cachectin
Protein/peptide type
Recombinant
Protein Function
Cytokine
Species
Mouse
Source
E. coli
Conjugation
Unconjugated
Gene ID
P06804
Sequence
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
Amino acid number
157
Protease-free
N
Animal-free
Y
Nuclease-free
N
Carrier-free
Y
Endotoxin-free
N
Endotoxin level
Low
Endotoxin content
Endotoxin LAL (EU/µg) ≤0.1
Biological activity
Bioactivity Assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D, ED₅₀ ≤0.1 ng/ml, Bioactivity ≥1.0×10⁷ units/mg
Purity
≥95%
Grade
RUO
Formulation
10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Molecular weight
17.4 kDa
Reconstitution instructions
Sterile water at 0.1 mg/ml
Storage conditions
–20 °C
Shipping temperature
RT
Tested applications
Bioassay
77660-832
Vial
100 µg
Log in for availability
Log in for availability
Each
Each - $848.92
$848.92
Each
$848.92
Minimum order quantity is 1
Supplier Number: 200-31AF-100UG
Specifications:
More
Specifications
Cat. No.
77660-832
Protein/peptide name
TNF-A (AF)
Protein synonyms
Cytotoxin|DIF|Necrosin|Cachexin|TNFSF2|TNF|Cachectin
Protein/peptide type
Recombinant
Protein Function
Cytokine
Species
Mouse
Source
E. coli
Conjugation
Unconjugated
Gene ID
P06804
Sequence
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
Amino acid number
157
Protease-free
N
Animal-free
Y
Nuclease-free
N
Carrier-free
Y
Endotoxin-free
N
Endotoxin level
Low
Endotoxin content
Endotoxin LAL (EU/µg) ≤0.1
Biological activity
Bioactivity Assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D, ED₅₀ ≤0.1 ng/ml, Bioactivity ≥1.0×10⁷ units/mg
Purity
≥95%
Grade
RUO
Formulation
10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Molecular weight
17.4 kDa
Reconstitution instructions
Sterile water at 0.1 mg/ml
Storage conditions
–20 °C
Shipping temperature
RT
Tested applications
Bioassay
77660-838
Vial
500 µg
Log in for availability
Log in for availability
Each
Each - $3,646.64
$3,646.64
Each
$3,646.64
Minimum order quantity is 1
Supplier Number: 200-31AF-500UG
Specifications:
More
Specifications
Cat. No.
77660-838
Protein/peptide name
TNF-A (AF)
Protein synonyms
Cytotoxin|DIF|Necrosin|Cachexin|TNFSF2|TNF|Cachectin
Protein/peptide type
Recombinant
Protein Function
Cytokine
Species
Mouse
Source
E. coli
Conjugation
Unconjugated
Gene ID
P06804
Sequence
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
Amino acid number
157
Protease-free
N
Animal-free
Y
Nuclease-free
N
Carrier-free
Y
Endotoxin-free
N
Endotoxin level
Low
Endotoxin content
Endotoxin LAL (EU/µg) ≤0.1
Biological activity
Bioactivity Assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D, ED₅₀ ≤0.1 ng/ml, Bioactivity ≥1.0×10⁷ units/mg
Purity
≥95%
Grade
RUO
Formulation
10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Molecular weight
17.4 kDa
Reconstitution instructions
Sterile water at 0.1 mg/ml
Storage conditions
–20 °C
Shipping temperature
RT
Tested applications
Bioassay
77660-834
Vial
1 mg
Log in for availability
Log in for availability
Each
Each - $6,085.59
$6,085.59
Each
$6,085.59
Minimum order quantity is 1
Supplier Number: 200-31AF-1MG
Specifications:
More
Specifications
Cat. No.
77660-834
Protein/peptide name
TNF-A (AF)
Protein synonyms
Cytotoxin|DIF|Necrosin|Cachexin|TNFSF2|TNF|Cachectin
Protein/peptide type
Recombinant
Protein Function
Cytokine
Species
Mouse
Source
E. coli
Conjugation
Unconjugated
Gene ID
P06804
Sequence
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
Amino acid number
157
Protease-free
N
Animal-free
Y
Nuclease-free
N
Carrier-free
Y
Endotoxin-free
N
Endotoxin level
Low
Endotoxin content
Endotoxin LAL (EU/µg) ≤0.1
Biological activity
Bioactivity Assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D, ED₅₀ ≤0.1 ng/ml, Bioactivity ≥1.0×10⁷ units/mg
Purity
≥95%
Grade
RUO
Formulation
10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Molecular weight
17.4 kDa
Reconstitution instructions
Sterile water at 0.1 mg/ml
Storage conditions
–20 °C
Shipping temperature
RT
Tested applications
Bioassay