Specifications
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Mouse
- Target protein:AST
- Size:1 kit
- Sample type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Cross reactivity:No significant cross-reactivity or interference between Low Density Lipoprotein Receptor Related Protein 1 (LRP1) and analogues was observed
- Detection method:Colorimetric
- Time to Results:3 h
- Shelf life:12 Months
- Detection range:1.56 - 100 ng/ml
- Storage temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample volume:100 µl
- Sensitivity:0.64 ng/ml
- Regulatory status:RUO
- Cat. no.:MSPP-SEB214MU
- No. of Tests:96 wells
Specifications
About this item
This assay has high sensitivity and excellent specificity for detecting Mouse Aspartate Aminotransferase (AST). The assay range is from 1.56 to 100 ng/ml (Sandwich kit) with a sensitivity of 0.62 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-assay: CV<10%; Inter-assay: CV<12%
AST) is similar to alanine transaminase (ALT) in that it is another enzyme associated with liver parenchymal cells.It is raised in acute liver damage. It is also present in red blood cells and cardiac muscle, skeletal muscle, and kidney and brain tissue, and may be elevated due to damage to those sources as well.AST was defined as a biochemical marker for the diagnosis of acute myocardial infarction in 1954. However the use of AST for such a diagnosis is now redundant and has been superseded by the cardiac troponins. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic.