phi29-XT WGA Kit
About this item
The phi29-XT WGA Kit has been optimized for sensitive, fast, and robust whole genome amplification (WGA), an isothermal method for amplification of an entire genome, starting from femtogram quantities of DNA and resulting in microgram quantities of amplified products.
- High sensitivity: Amplify from as little as 10 fg of input DNA
- Powered by phi29-XT DNA Polymerase, an engineered polymerase
- Flexible protocol allows for different types of input material
- Simple workflow produces high yield in a short reaction time (less than 2 hours)
- Whole genome representation and uniform GC coverage for human and microbial DNA
The kit features phi29-XT DNA polymerase, an engineered polymerase with improved thermostability, sensitivity, and generates higher yield in a shorter reaction time than wild-type phi29 DNA Polymerase. Also included are exonuclease-resistant random primers (containing phosphorothioate bonds) to universally amplify any DNA sequence, neutralization buffer, and dNTPs.
Input material can be purified genomic DNA, cells, or microbiomes. WGA enables several downstream applications, including single cell whole genome sequencing (WGS), genomic DNA enrichment, and environmental DNA (eDNA) enrichment and sequencing.
2 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
The phi29-XT WGA Kit has been optimized for sensitive, fast, and robust whole genome amplification (WGA), an isothermal method for amplification of an entire genome, starting from femtogram quantities of DNA and resulting in microgram quantities of amplified products.
- High sensitivity: Amplify from as little as 10 fg of input DNA
- Powered by phi29-XT DNA Polymerase, an engineered polymerase
- Flexible protocol allows for different types of input material
- Simple workflow produces high yield in a short reaction time (less than 2 hours)
- Whole genome representation and uniform GC coverage for human and microbial DNA
The kit features phi29-XT DNA polymerase, an engineered polymerase with improved thermostability, sensitivity, and generates higher yield in a shorter reaction time than wild-type phi29 DNA Polymerase. Also included are exonuclease-resistant random primers (containing phosphorothioate bonds) to universally amplify any DNA sequence, neutralization buffer, and dNTPs.
Input material can be purified genomic DNA, cells, or microbiomes. WGA enables several downstream applications, including single cell whole genome sequencing (WGS), genomic DNA enrichment, and environmental DNA (eDNA) enrichment and sequencing.
Product Family Options
Product Information
- No. of reactionsAvailabilityPrice
- 77591-838phi29-XT WGA kitSupplier Number: E1604SItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- CA77591-838phi29-XT WGA kitphi29-XT WGA kit25Discontinued-Supplier Number: E1604SItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:MarketsourceMarketsource
- 77591-836phi29-XT WGA kitSupplier Number: E1604LItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- CA77591-836phi29-XT WGA kitphi29-XT WGA kit100Discontinued-Supplier Number: E1604LItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:MarketsourceMarketsource
#: CA21905-026
Supplier: Kimberly-Clark