The PerFix-nc kit (no centrifuge assay Kit) has been developed to enhance the signal-to-noise ratio of intracellular staining and simplify the workload necessary for the sample preparation. Accurate detection of both intracellular and extracellular epitopes are obtained, while there are no washing steps through the whole straight procedure (only a final wash step is described as optional).

Several surface markers can be added together with the intracellular markers and incubated simultaneously during the permeabilization step. Total duration of the procedure and total workload are similar to current procedures for surface staining (e.g. VersaLyse + fix no wash), and much shorter than common permeabilization procedures. Potential automation of the PerFix-nc straight procedure is rendered possible thanks to the removal of the washing steps.