Specifications

• Pack type:
  Vial
• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  E. coli
• Species:
  Human
• Size:
  10 µg
• Storage conditions:
  4 °C
• Endotoxin content:
  <1 EU/μg of rHuLIF as determined by LAL method
• Biological activity:
  Fully biologically active when compared to standard. The specific activity determined by inducing differentiation of murine M1 myeloid leukemic cells. The minmun detectable concentration of rHuLIF in this assay is 0.5 ng/ml. The specific activity of >2×10⁸ IU/mg, where 50 units is defined as the amount of rHuLIF required to induce differentiation in 50% of the M1 colonies in 1 ml agar cultures.
• Reconstitution instructions:
  We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1 - 1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤−20 centigrade. Further dilutions should be made in appropriate buffered solutions.
• Endotoxin-free:
  No
• Carrier-free:
  No
• Protease-free:
  No
• Animal-free:
  No
• Protein synonyms:
  leukemia inhibitory factor
• UniProtKB:
  P15018
• Protein/peptide name:
  LIF
• Purity:
  >98% by SDS-PAGE and HPLC analyses
• Molecular weight:
  19.7 kDa
• Sequence:
  SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
• Endotoxin level:
  Low
• Nuclease-free:
  No
• Grade:
  Ultrapure grade
• Shipping temperature:
  20
• Cat. no.:
  77540-136