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Human Recombinant FCGR1A (from HEK293 cells)
  77539-450
 :  Creative Biomart
 :  
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Human Recombinant FCGR1A (from HEK293 cells)
  77539-450
 :  Creative Biomart
 :  FCGR1A-2224H
 :  

 

  • Pack type:
    Vial
  • Conjugation:
    Unconjugated
  • Protein/peptide type:
    Recombinant
  • Source:
    HEK293 cells
  • Species:
    Human
  • Size:
    50 µg
  • Storage conditions:
    4 °C
  • Endotoxin content:
    <1.0 EU/µg of the protein as determined by the LAL method
  • Biological activity:
    Immobilized Rituximab at 1 μg/ml can bind Human FcγRI/CD64 recombinant protein, with a linear range of 0.5 - 4.0 ng/ml. Captured Human FcγRI/CD64 recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 5.5 nM as determined in an SPR assay (Biacore T200). Captured Human FcγRI/CD64 recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 13.6 nM as determined in an SPR assay (Biacore T200). Loaded Human FcγRI/CD64 recombinant protein on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 8.5 nM as determined in a BLI assay (ForteBio Octet Red384). Loaded Human FcγRI/CD64 recombinant protein on His1K biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 4.7 nM as determined in a BLI assay (ForteBio Octet Red384). Loaded Rituximab (IgG1) on ProA biosensor, can bind Human FcγRI/CD64 recombinant protein with an affinity constant of 0.38 nM as determined in a BLI assay (ForteBio Octet Red384).
  • Reconstitution instructions:
    It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 µg/µl. Centrifuge the vial at 4 °C before opening to recover the entire contents.
  • Endotoxin-free:
    No
  • Carrier-free:
    No
  • Protease-free:
    No
  • Animal-free:
    No
  • Protein synonyms:
    Fc fragment of IgG, high affinity Ia, receptor (CD64)
  • UniProtKB:
    P12314
  • Protein/peptide name:
    FCGR1A
  • Purity:
    >95% as determined by SDS-PAGE. >90% as determined by SEC-HPLC.
  • Molecular weight:
    The recombinant human CD64A consists of 284 amino acids and has a predicted molecular mass of 32 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the recombinant human CD64A is approximately 45 - 60 kDa due to glycosylation.
  • Endotoxin level:
    Low
  • Nuclease-free:
    No
  • Grade:
    Ultrapure grade
  • Shipping temperature:
    20
  • Cat. no.:
    77539-450