Recombinant Human C3 (from HEK293 cells)

Recombinant Human C3 (from HEK293 cells)

Conjugation:
Unconjugated
Protein/peptide type:
Recombinant
Source:
HEK293 cells
Species:
Human
Storage conditions:
4 °C
Endotoxin content:
<1.0 EU/μg by the LAL method
Biological activity:
Immobilized human complement C3, his tag at 1 μg/ml (100 μl/well) can bind human anti-C3, human IgG1 human kappa with a linear range of 0.1 - 4 ng/ml.
Reconstitution instructions:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 µg/µl. Centrifuge the vial at 4 °C before opening to recover the entire contents.
Endotoxin-free:
No
Carrier-free:
No
Protease-free:
No
Animal-free:
No
Protein synonyms:
complement component 3
UniProtKB:
P01024
Protein/peptide name:
C3
Purity:
>95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
Molecular weight:
This protein carries a polyhistidine tag at the C-terminus the protein has a calculated MW of 186.8 kDa. The protein migrates as 70 kDa, 100 - 120 kDa and 160 - 180 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin level:
Low
Nuclease-free:
No
Grade:
Ultrapure grade
Shipping temperature:
20