Anti-CD25 Rat Monoclonal Antibody [clone: 3C7]

MSPP-60010

Anti-CD25 Rat Monoclonal Antibody [clone: 3C7]

MSPP-60010

: 60010

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Antigen name:
CD25
Clonality:
Monoclonal
Clone:
3C7
Host:
Rat
Isotype:
IgG2b kappa
Reactivity:
Mouse
Size:
500 µg
Gene ID:
16184
Cat. no.:
MSPP-60010

Rat monoclonal IgG2b antibody against mouse CD25, unconjugated.

T cells
T cells Regulatory

The 3C7 antibody reacts with murine CD25 (interleukin‐2 receptor α chain; IL‐2Rα), an ~55 kDa type 1 transmembrane glycoprotein expressed on the surface of T and B cell progenitors and activated mature B and T cells. CD25 has been used as a marker to identify CD4+/FoxP3+ regulatory T cells in mice. Mutations in CD25 result in severe immunodeficiency, underscoring the importance of the roles the protein plays in the differentiation, activation and proliferation of lymphocytes, and in the maintenance of self-tolerance. CD25 per se has low affinity for its IL-2 ligand but forms dimers with CD122 (IL-2Rβ) and CD132 (IL-2Rγ) that together associate to form the IL-2R receptor, which binds IL-2 with high affinity. CD25 acts to increase the specificity and affinity of IL-2 binding and is necessary for receptor clustering and signal transduction by the complex. Binding of the 3C7 antibody inhibits binding of IL‐2 to free CD25 and the IL-2R receptor. The epitope is distinct from those recognized by the anti-CD25 antibody clones 7D4 and PC61. This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ mouse T cell enrichment kit (Catalog #19751).

: Product restrictions: (i) Customer shall not make products or any portion of them in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier and (ii) Customer is familiar with Supplier’s restrictions on the use of the products and shall use the products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').

Type: Anti-CD25 Rat Monoclonal Antibody [clone: 3C7]
Antigen: CD25
Clonality: Monoclonal
Clone: 3C7
Conjugation: Unconjugated
Epitope:
Host: Rat
Isotype: IgG2b kappa
Reactivity: Mouse