This mixture contains seven purified proteins that range in size from 14 to 120kDa

The buffer composition is 62.5mM TRIS-HCl at pH 6.8, 2% (w/v) SDS, 250mM sodium chloride, 1mM EDTA, 4% (v/v) [beta]-mercaptoethanol, 35% (v/v) glycerol, and 0.05% (w/v) bromophenol blue with 0.01% (w/v) sodium azide as a preservative. Store marker mixture at –20°C (–4°F).

It has been formulated to yield well-defined bands after SDS-PAGE electrophoresis and staining with Coomassie blue or other common staining techniques.The bovine albumin band (67kDa) has been increased to serve as a reference. It is supplied in 1X loading buffer, so that it is ready to use.