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Specifications
- Description:Aqueous Compatible Formulation Kit
- Cat. no.:CAPI23280
- No. of Assays/Kit:100 tube assays or 500 microplate assays with non-lipid samples
- Supplier No.:23280
Specifications
About this item
Pierce™ Quantitative Peroxide Assay Kit (Aqueous) detects and measures hydrogen peroxide levels in biological samples using an Iron and Xylenol orange* (XO) reagent.
- Ready to use – removes the variability, difficulty and expense of formulating in-house
- Ideal for sensitive measurement of peroxide accumulation in non-lipid samples
The Aqueous compatible peroxide assay kit detects peroxides based on oxidation of ferrous to ferric ion in the presence of xylenol orange
Peroxides in the sample oxidize Fe2+ to Fe3+. The Fe3+ ion will form a colored complex with xylenol orange that can be read at 560nm.
*Xylenol orange is (o-cresolsulfonephthalein-3´-3´-bis-[methyliminodiacetic acid sodium salt]).
The kit formulation includes sorbitol, which provides sensitivity enhancement. Most proteins do not interfere with this assay, although some metal chelators may require use of a blank (ie. control) to mitigate these effects.