Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/ca/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
- Protein delivery:Yes
- Description:Pierce Pro-Ject™ Protein Transfection Reagent
- Cat. no.:CAPI89850
- Volume:250 µl
- Supplier No.:89850
This Pierce™ protein transfection reagent is a cationic lipid mixture for complexation with proteins, peptides, antibodies and other biologically active molecules to allow their direct intracellular delivery.
- No need to isolate, clone and transfect gene sequences
- Optimum delivery in three to four hours after incubation
- Delivery complex is non-covalent
- Works with proteins, peptides and fluorescently labeled antibodies
The reagent/protein complexes offer optimum delivery within three to four hours after incubation and the complex is noncovalent so it does not interfere with biological activity
Molecules that have been successfully delivered with the transfection reagent include β-galactosidase, fluorescent-antibody, green fluorescent protein (GFP), high and low molecular weight dextran sulfate, phycoerythrin-BSA, caspase 3, caspase 8 and granzyme B.
It attaches to negatively charged cell surfaces and enters the cell either by directly fusing with the plasma membrane or by enodcytosis and subsequent fusion with the endosome. The transfection reagent may be used to study intercellular signaling, cell cycle regulation, apoptosis, oncogenesis, and transcriptional regulation.