TeSR™-AOF 3D Medium

Animal origin-free suspension culture medium for human ES and iPS cells.

TeSR™-AOF 3D is an animal origin-free culture medium developed for expansion and scale-up of undifferentiated human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells as aggregates in 3D suspension culture. Based on TeSR™-AOF, TeSR™-AOF 3D is a robust medium with an optimized fed-batch protocol for scaling up human ES and iPS cell production in suspension culture. The novel fed-batch workflow saves time and media; daily feeds replenish nutrients and eliminate the need for medium exchanges on non-passaging days. The TeSR™-AOF 3D system is compatible with cells previously cultured in mTeSR™1, mTeSR™ plus, TeSR™-AOF, or TeSR™-E8™ on Corning® Matrigel® hESC-qualified matrix, Vitronectin XF™, or CellAdhere™ Laminin-521. TeSR™-AOF 3D is compatible with a range of suspension culture vessels. No materials of animal or human origin are used in the manufacture of this medium or its components, to at least the secondary level of manufacturing. TeSR™-AOF 3D is animal component-free to the secondary level. Each lot of TeSR™-AOF 3D seed or feed supplement that is used to prepare complete TeSR™-AOF medium is performance-tested in a culture assay using human pluripotent stem cells.

 : Product restrictions: (i) Customer shall not make products or any portion of them in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier and (ii) Customer is familiar with Supplier’s restrictions on the use of the products and shall use the products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').