Medium without cytokines.

Use MegaCult™-C medium without cytokines for the culture of colony-forming unit-megakaryocyte progenitor cells (CFU-Mk) in human bone marrow, mobilized peripheral blood, and cord blood samples, after addition of appropriate cytokines. Available either individually or as part of the MegaCult™-C collagen and medium without cytokines kit, this medium is suitable for use with CD34+ enriched cells, mononuclear cells, and cells isolated by other purification methods. It is also intended for assays of megakaryocyte progenitor cells in unseparated or purified cell suspensions of mouse bone marrow, after addition of appropriate cytokines. Required MegaCult™-C medium may be used with collagen solution, available either individually or as part of the MegaCult™-C collagen and medium without cytokines kit.