Perform one-step quantitative PCR assays with Radiant™ 1-Step SYBR Green Mastermix qPCR Kit, Hi-ROX, providing convenient and sensitive detection.

• Thermostable reverse transcriptase 45 to 55 ˚C
• Contains advanced RNase inhibitor
• Non-PCR inhibiting intercalating dye
• Rapid extension rate for early Ct values
• Market leading sensitivity - increased limit of detection
• Compatible on all real-time PCR platforms

Radiant™ 1-Step SYBR Green Kit uses the latest developments in reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The qPCR is performed by Radiant™ Taq HS DNA Polymerase, resulting in robust RT-PCR performance.

Radiant™ 1-Step SYBR Green Kit can be used to quantify any RNA template including mRNA, total RNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency. small molecular inhibitor technology prevents formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest advancements in polymerase technology and advanced buffer chemistry, offering excellent performance with minimal or no optimization.

Many modern real-time PCR instruments have a fast cycling mode. The same developments that improve the sensitivity and consistency of Radiant™ SYBR GREEN Kits in standard cycling conditions also allow for industry leading performance in fast and ultra fast cycling conditions.