Use BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk

ELISA microwell blocking buffer with stabilizer is designed to block microwells coated with antigens, antibodies, or other ligands and stabilize the plates for drying. Once plates are stabilized, they can be stored for up to one year without a significant loss of signal. The near infra-red blocking buffer is designed to be used with IRDye® 700Dx, IRDye 800, Alexa Fluor® 680, and Cy™5.5 antibody and reagent conjugates.

BLOTTO A is a ready-to-use general purpose blocking agent. It is 5% non-fat dry milk in TBS. BLOTTO B is recommended for use when phospho-specific antibodies are used. It is 1% non-fat dry milk in TBS.