The Total RNA Mini and Maxi Kits are specially designed for purification of total RNA from fresh whole human blood and cultured cells

This method uses detergents and a chaotropic salt to lyse cells and inactivate Rnase. The RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. Once any contaminants have been removed following the wash step, the purified RNA is eluted by RNase-free water. The entire procedure can be completed within 30 minutes (mini kits) and 60 minutes (maxi kits). The purified RNA is ready for RT-PCR, Northern Blotting, Primer Extension, and cDNA library construction.