Hepatic stellate cells are responsible for the regulation of extracellular matrix proteins and vascular structure in the liver. They represent approximately 5 to 8% of the cells in the liver and function to maintain homeostasis in the microenvironment of the hepatic sinusoid. They play a critical role in the development of liver fibrosis. When chronic liver damage occurs, hepatic stellate cells are actively synthesizing extracellular matrix proteins such as collagen and other glycoproteins. The accumulation of the extracellular matrix proteins trigger hepatic fibrosis which can lead to cirrhosis.

• Cryovial: ~0.5×10⁶ to ~1.0×10⁶

Tempo-iHepStellate™ are reprogrammed from human iPSCs, using integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary serum-free, virus-free, nucleic-acids-free, and integration-free conditions.

They express the following biomarkers using RT/q-PCR and Immunocytochemistry (ICC) methods: VIM, DES, ACTA-2, CYGB, PCDH7, NTF3, SYP, RBP1, LRAT, Lox, CDH1, NCAM, PDGFRB, (COL1A1 is positive, if co-culture includes Tempo-iHep3D™), and (PNPLA3 is positive, if co-cultured with Tempo-iHep3D™).

Intended for scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.

Long-term storage: liquid nitrogen
Growth Properties: 2D/monolayer cell culture technique; 3D/spheroid/organoid cell culture techniques
QC: Sterility, safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>85%)