CD9 (clone HI9a) is a validated mouse monoclonal antibody that recognizes the human tetraspanin protein CD9. The antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance.

This antibody has been validated in flow cytometry, in immunofluorescence microscopy, and in western blot. The antibody is available conjugated to R-PE or Astral™ tandem dyes. They are supplied in PBS, 0.1% BSA, 0.05% azide.

CD9 is a type IV transmembrane glycoprotein with four transmembrane domains. CD9 on pre-B cells may play a role in cell-cell adhesion. In addition, CD9 may play a role in signal transduction mediated by interaction with low molecular weight GTP binding proteins. CD9 is expressed on early B cells, eosinophils, basophils and activated T cells and is a major component of the platelet cell surface. It is also expressed on most non-T acute lymphoblastic leukemia cells and on some acute myeloid and chronic lymphoid leukemia.

Tetraspanin proteins CD9, CD63, and CD81, are the most common proteins used as exosome markers. Tetraspanins are plasma membrane proteins with many proposed functions, including activation and sorting of other membrane proteins. They are also thought to play a role in the targeting of proteins to multivesicular bodies (MVBs) and exosomes. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of exosomes, but their expression levels vary depending on the cell type of origin.

Type: Primary
Antigen: CD9
Clonality: Monoclonal
Clone: HI9a
Conjugation: RPE-Astral™616
Epitope:
Host: Mouse
Isotype: IgG1 kappa
Reactivity: Human, Bovine, Rabbit, Baboon, Cynomolgus monkey, Dog, Horse, Non-human primates, Sheep