CD16 (Clone 3G8) is a mouse monoclonal antibody that recognizes IgG receptor CD16, also known as FC gamma receptor III. This antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance. The antibody is available conjugated to CF® Dyes and Astral™ tandem dyes. They are supplied in PBS, 0.1% BSA, 0.05% azide.

• Cell/tissue expression: Lymphocytes
• SwissProt: P08637
• Unigene: 372679
• Positive control: Human peripheral blood mononuclear cells (PBMCs)
• Ex/Em: 496, 546, 566/617 nm
• Storage condition: Protect fluorescent conjugates from light

This antibody recognizes CD16 (Fc gamma receptor III), the low-affinity receptor for IgG with an apparent molecular weight of 50 to 80 kDa. CD16 is represented by two similar genes, CD16A (Fc gamma receptor IIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gamma receptor IIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1.

CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

Shelf life: Guaranteed for at least 24 months from date of receipt when stored as recommended.

Type: Primary
Antigen: CD16
Clonality: Monoclonal
Clone: 3G8
Conjugation: RPE-Astral™616
Epitope:
Host: Mouse
Isotype: IgG1 kappa
Reactivity: Human