The NEXTFLEX® 16S V4 Amplicon-Seq Kit 2.0 is designed to prepare multiplexed amplicon libraries that span the fourth hypervariable domain of microbial 16S ribosomal RNA (rRNA) genes.

• Fast and easy library prep protocol
• Low input – Only 1 ng of input required
• Multiplex up to 384 libraries
• Custom sequencing primers are not required
• Automation protocols are now available for the Sciclone® NGS and NGSx Workstations to automate your 16S sequencing
• Functionally tested with Illumina® sequencing platforms

Optimized Protocol Offers Lower PCR Bias and Fewer Off-target Reads
The NEXTFLEX 16S V4 Amplicon-Seq Kit 2.0 incorporates a second PCR step in the protocol for the addition of the sample-specific index which reduces off-target reads encountered during amplicon sequencing.

384 Barcodes Available for Cost-effective 16S V4 rRNA Sequencing
The NEXTFLEX 16S V4 Amplicon-Seq Library Prep Kit 2.0 can be used for multiplexing up to 384 samples, to greatly reduce the cost of sequencing. The barcodes are offered in sets of 4, 12, 48 or 96 barcodes and supplied with 2 reactions worth of each barcode.

Automate your 16S V4 Library Prep to Increase Throughput and Reduce Errors
A NEXTFLEX® automation protocol is now available on the Sciclone® NGS and NGSx workstations to help labs increase their throughput and reduce human errors.

A NEXTFLEX® automation protocol is now available on the Sciclone® NGS and NGSx workstations to help labs increase their throughput and reduce human errors.

The NEXTFLEX 16S V4 rRNA Amplicon-seq panels are now available bundled with access to CosmosID-HUB; an online software solution that enables fast and easy analysis of complex microbiome data. CosmosID-HUB gives scientists user-friendly access to version-controlled and validated 16S pipelines. The machine-learning powered software also enables rapid data interpretation through a comparative analysis software with features such as tables, heatmaps, bar charts, multiple Alpha & Beta Diversity indexes, abundance distribution plots, differential abundance testing, as well as comprehensive statistics between groups. Learn more about CosmosID-HUB.