The NEXTFLEX® UDI Barcodes (1 to 1536) are 1536 unique dual index adapters with 10 nt indexes for multiplexing of NGS libraries compatible with Illumina® sequencers.

• 384 UDI adapters for mitigating sample misassignment due to index hopping
• Unique dual index adapters for multiplexing up to 384 libraries for Illumina® sequencing platforms
• Mitigate index hopping or spread of signal that can occur during sequencing
• Each lot of the NEXTFLEX® unique dual index barcodes is tested for index purity by sequencing
• Compatible with both paired-end and single-read sequencing
• Compatible with the NEXTFLEX® universal blockers

The NEXTFLEX unique dual index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction.

Each NEXTFLEX unique dual index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina NovaSeq®, HiSeq® 3000/4000, and HiSeq X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing.

The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is tested for index purity and functionality by sequencing. The design of the NEXTFLEX unique dual index barcodes allow a broad range of multiplexing - users can multiplex anywhere between two samples and 1536 samples in a single sequencing run.