The NEBNext® enzymatic 5hmC-seq kit specifically detects 5hmC sites.

• 5hmC detection at single base resolution
• Enzyme-based conversion minimizes DNA damage
• 0.1 to 200 ng input range
• Even GC coverage
• E5hmC-seq and EM-seq data can be combined

The kit includes NEBNext Ultra II library prep reagents, and 5hmC is detected using a two-step enzymatic conversion workflow that minimizes damage to DNA and allows discrimination of 5hmC from both cytosine and 5mC after Illumina sequencing. E5hmC-seq data can also be subtracted from EM-seq data, allowing determination of the precise location of individual 5mC and 5hmC sites. The kit includes the E5hmC-seq adaptor and index primers are supplied separately.