For quantitative determination of FTase enzyme activity in biological samples.

• Safe and convenient
• Non-radioactive assay
• 'Mix-incubate-measure' type assay
• No wash and reagent transfer steps are involved
• Sensitive and accurate
• Linear detection range 0.024 to 3.2 U/L FTase in a 384-well plate assay
• High-throughput
• Can be readily automated to assay thousands of samples per day

FARNESYLTRANSFERASE (FTase, EC 2.5.1.58) catalyzes the transfer of a farnesyl group from farnesyl pyrophosphate to the cysteine residue of the C-terminus of target proteins. When not properly regulated, farnesylated proteins, including the Ras superfamily of small GTPases, can lead to developmental disorders and cancer. Simple, direct and high-throughput activity assays find wide applications for cancer research, while BioAssay Systems’ EFTS-400 assay kit provides a convenient fluorimetric method for assaying FTase activity. In this assay, FTase reacts with farnesyl pyrophosphate and the dansyl-peptide substrate releasing a product that is measurable by fluorescence (λem/ex = 340/550 nm).