For quantitative determination of plasmin enzyme activity in biological samples.

• Safe and sensitive
• Non-radioactive assay
• Use as little as 10 μl samples
• Linear detection range in 96-well plate: 0.35 to 56.5 U/L activity
• Fast and convenient
• The procedure involves addition of a single working reagent and incubation for 30 min
• High-throughput
• Homogeneous 'mix-incubate-measure' type assay
• Can be readily automated to assay thousands of samples per day

PLASMIN (3.4.21.7) is a serine protease that is a key enzyme in the process of fibrinolysis. Plasmin is released into circulation as its inactive form, plasminogen, which can be activated by enzymes such as urokinase. The plasmin/plasminogen system has been studied for its role in inflammation, degradation of the extracellular matrix, and wound healing. BioAssay Systems’ DPLM-100 kit provides a convenient colorimetric method to measure plasmin activity. In this assay, plasmin hydrolyzes a synthetic substrate to release p-nitroanilide (pNA), which absorbs at 405 nm. The increase in absorbance at 405 nm is directly proportional to the enzyme activity.