HRP determination in ELISA, Southern Blot, Western Blot.

• Single reagent: Single, stable, and ready to use
• High sensitivity: Detection of HRP in the atto- to low femtogram/well range

Peroxidases (or peroxide reductases, EC number 1.11.1.x) are a large group of enzymes that break up peroxides and play important roles in various biological processes. Horse Radish Peroxidase (HRP) is an enzyme commonly used for conjugation. One example is antibody-HRP conjugate that is frequently and widely used in immunoassays such as ELISA and Western blots.

BioAssay systems SuperLight™ atto HRP reagent provides an ultra-sensitive luminescent means for detecting HRP activity and its conjugates. The improved reagent rapidly reacts with peroxidase or the HRP in an HRP-conjugate, producing highly stable and intense luminescence for maximum detection of the peroxidase activity in solution assays.