For quantitative determination of urokinase activity determination in biological samples.

• Safe and non-radioactive assay
• Fast
• Assay is completed within a 15 minute reaction time
• Homogeneous 'mix-incubate-measure' type assay
• Can be readily automated to assay thousands of samples per day

Urokinase plasminogen activator (urokinase, uPA) is a key serine protease involved in the degradation of the extracellular matrix that catalyzes the conversion of plasminogen to active plasmin. It acts as a thrombolytic agent to break up blood clots and when over-expressed, has been reported to influence the growth of certain malignant tumors (breast, prostate, etc).

BioAssay systems DUKN-100 kit provides a convenient fluorimetric method to measure urokinase activity in biological samples. In this assay, the fluorimetric substrate reacts with urokinase so that the increase in fluorescence at λex/em = 380/450 nm is directly proportional to enzyme activity.