AASTY Screening Kit

Each membrane protein has its native lipid environment. Therefore, each lipid environment is unique to each membrane protein. Moreover, it is this lipid environment that synthetic polymers like AASTYs interact with. Therefore, one needs a broader selection of polymers since there is not one AASTY that fits each phospholipid composition. To address this question, AASTYs come in three different ratios of acrylic acid to styrene. The ratios differ in hydrophilic property of AASTY, which results in different affinities to varying membrane lipid compositions. The second feature to screen for when it comes to AASTY is the size of the resulting nanodiscs. One of AASTY's most excellent features, compared to SMA or DIBMA, is that its nanodiscs are pretty uniform in size. Therefore, the correct fitting nanodisc size must be determined before.

AASTY 6-45:
State: Lyophilized Powder, to be solved with water
Molecular weight: 5-6 kDa
Buffer: HEPES
pH after solving: 7.5
Divalent cationic tolerance: 6 mM
Acrylic Acid to Styrene Ratio:45% to 55%

AASTY 11-45
Molecular weight: 5-6 kDa
Buffer: HEPES
pH after solving: 7.5
Divalent cationic tolerance: 6 mM
Acrylic Acid to Styrene Ratio: 45% to 55%

AASTY 6-50:
Molecular weight: 10-11 kDa
Buffer: HEPES
pH after solving: 7.5
Divalent cationic tolerance: 6 mM
Acrylic Acid to Styrene Ratio: 50% to 50%

AASTY 11-50
Molecular weight: 10-11 kDa
Buffer: HEPES
pH after solving: 7.5
Divalent cationic tolerance: 6 mM
Acrylic Acid to Styrene Ratio: 50% to 50%

AASTY 6-55:
Molecular weight: 5-6 kDa
Buffer: HEPES
pH after solving: 7.5
Divalent cationic tolerance: 6 mM
Acrylic Acid to Styrene Ratio:55% to 45%

AASTY 11-55:
Molecular weight: 10-11 kDa
Buffer: HEPES
pH after solving: 7.5
Divalent cationic tolerance: 6 mM
Acrylic Acid to Styrene Ratio: 55% to 45%

Caution: Shipping Temperature: Ambient temperature. Storage of lyophilized copolymer: -20°C for several years. Storage of dissolved copolymer: 2-8°C for several day.