SARS-CoV-2 Recombinant SARS-CoV-2 Spike Protein in SMALP 200 (from HEK293 cells)
Catalog # 77392-864
Supplier: CUBE BIOTECH
CAS Number:
Specifications
- Protein/peptide type:Recombinant
- Source:HEK293 cells
- Species:SARS-CoV-2
- Size:25 µg
- Environmentally Preferable:
- Storage conditions:−80 °C, avoid freeze-thaw cycles
- Amino acid number:1286 (Including Rho1D4-tag and linker)
- Protein synonyms:Spike glycoprotein
- Protein/peptide name:SARS-CoV-2 Spike Protein in SMALP 200
- Purity:>98%, determined via SDS-PAGE
- Molecular weight:142114 Da
- Shipping temperature:Dry Ice
- Cat. no.:77392-864
Specifications
About this item
The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.
- Alternative names: SPIKE_SARS2 Spike glycoprotein
- UniProt Number: P0DTC2
- Protein class: Single span transmembrane protein
- Organism: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
- Sequence, explained: Full-length sequence (aa 1 - 1273), furin cleavage site 'RRAR' mutated to 'GSAG'; C-terminal Rho1D4 tag fused with spacer 'GSSG' to protein sequence
- Affinity tags: C-terminal Rho1D4-tag
- Expression Host: Hek293 Expi cells
- Buffer composition: 20 mM Hepes pH 7.5, 150 mM NaCl, buffer does not contain free SMALP 200
- Stabilization agent: SMALP 200
- Purified via: PureCube Rho1D4 Agarose
- Function: Host cell surface receptor binding; fusion of virus membrane with host endosome membrane
The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE this trimer needs to be kept intact. And Cube Biotech offers stabilized intact form of SPIKE.