Our PureCube Ni-IDA XL agarose resins are agarose resin beads with a diameter of ∼400 µm. They are used to purify active His-tagged proteins from cells and extremely viscous cell media.

• Usage: Specific binding and purification of 6× His tagged proteins
• Specificity: Affinity to His tagged proteins
• Binding capacity: >30 mg/ml
• Bead ligand: Ni-IDA
• Bead size: 400 μm
• Filling quantity: Delivered as a 50% suspension
• pH stability: 2 to 14
• Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile

Our year-long experience in manufacturing agarose resin led to the high yield of 20 mg protein per ml resin, leading in the market compared to other Ni-IDA suppliers. PureCube Ni-IDA resins are suited for batch spin columns and FPLC. Their small diameter provides them with excellent mechanical stability.

Required equipment:
-Lysis buffer
-Wash Buffer
-Elution buffer
-Ice bath
-Refrigerated centrifuge for 50 ml tube (min 10000×g)
-50 ml centrifuge tube
-Micropipettor and Micropipetting tips
-Disposable gravity flow columns with capped bottom outlet, 2 ml
-pH meter
-End-over-end shaker
-SDS-PAGE buffers, reagents and equipment; Optional: Western Blot reagents and equipment