This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

• Origin of protein: Human
• Purity: >90% (Determined by SDS-PAGE)
• Number of amino acids: >217
• Molecular mass: 25309 Da
• Extinction coefficient (in water) ε280: 25440 M⁻¹cm⁻¹
• Buffer: 20 mM TRIS pH 7.4, 100 mM NaCl, 0.5 mM EDTA
• Sequence (1-Letter code): MGSSHHHHHH ENLYFQ GLKLLDNWDSVTSTFSKLREQLG PVTQEFWDNLE KETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFK VSFLSALEEY TKKLNTQ
• Affinity tag: N-Terminal His-tag

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1D1 dH5 (His-tagged) creates nanodiscs of ∼7 to 8 nm in diameter.

This product contains a His-tag, keep that in mind when choosing the affinity tag for your membrane protein.