PureCube Zr-NTA MagBeads/magnetic beads were developed for the enrichment of phosphorylated proteins. They can also be used for the purification of His-tagged proteins as a secondary function.

• Usage: Enrichment of phosphopeptides, specific binding and purification of 6× His-tagged proteins
• Specificity: Affinity to His-tagged proteins
• Affinity to phosphorylated biomolecules
• Bead ligand: Fe-NTA
• Bead size: 40 µm
• Filling quantity: Delivered as a 50% suspension
• Matrix: 7.5% crosslinked agarose
• Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
• pH stability: 2 to 14
• Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile

The affinity matrix is based on 6% cross-linked agarose that was magnetized. The bead ligand is Zr-NTA and the particle diameter is 30 µm. They are very homogeneous in size, yielding in a high degree of reproducibility. The phosphopeptide enrichment with Cube Biotech MagBeads can be automated as it has already been proven in this paper.