The HisCube MagBead protein purification MINI kit is designed to meet the demands of your laboratory for efficient and high-quality small-scale His-tag protein purifications.

• Usage: Enrichment of phosphopeptides, specific binding and purification of 6× His-tagged proteins
• Specificity: Affinity to phosphorylated biomolecules
• Bead ligand: Zr-NTA
• Bead size: 30 µm
• Filling quantity: Delivered as a 25% suspension
• Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
• pH stability: 2 to 14
• Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile

Our team of experts has put their extensive knowledge and experience in His-tag purifications into developing this kit, resulting in a streamlined protocol that is easy to follow. The included MagBead separator is a testament to our commitment to delivering a comprehensive and user-friendly solution. The HisCube INDIGO-Ni MagBead MINI kit contains our in-house developed PureCube INDIGO Ni-MagBeads.

The significant benefit of INDIGO is its tolerance for chelating and reducing agents. While these can significantly assist His-tag purifications, conventional His-tag affinity beads do not tolerate them. EDTA is a chelator often used in eukaryotic cell buffers because it has protease-inhibiting functions. DTT can dissolve protein aggregates that could potentially hinder the access of the purification beads to the His-tagged proteins. However, both chemicals are now usable because of INDIGO. As a result, they are a part of the kit.