The PureCube Ni-INDIGO column/cartridge is an IMAC chromatography column prepacked with PureCube 100 INDIGO-Ni Agarose resin beads.

• Usage: Specific binding and purification of 6× His tagged proteins
• Specificity: Affinity to His-tagged proteins
• Binding capacity: >100 mg/ml
• Bead Ligand: Ni-INDIGO
• Bead size: 100 μm
• Filling quantity: Delivered as a 50% suspension
• pH stability: 2 to 14
• Chelator stability (e.g. EDTA): 20 mM
• Reducing agent stability (e.g. DTT): 20 mM
• Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile

Features - PureCube mini batch spin column:
Body material: Polypropylene
Filter pore size: 0.1 to 0.2 µm low binding PVDF
Max. volume: 20 ml
Max. g force: 1500 to 2000×g (swing-bucket rotor)
Min. g force: 100 to 200×g for 1 to 10 min

You can choose between two sizes, 1 ml bed volume and 5 ml bed volume. The PureCube cartridge/column that is used here is compatible with almost all commonly used FPLC systems like ÄKTA. We further recommend checking out our other Ni-INDIGO products.