HighSpec Rho1D4 agarose XL resin is a suspension of 400 µm agarose beads coupled to an anti-Rho1D4 antibody.

• Usage: Coupling biomolecules to active carboxy groups
• Specificity: Exposed amine (NH₂) groups on biomolecule
• Amine group density: Higher than 15 µmol/ml
• Bead ligand: Chemically active Carboxy groups
• Bead size: 90 μm
• Filling quantity: Delivered as a 25% suspension
• Matrix: 6% cross-linked agarose
• Short term storage: In equilibration buffer (see protocol)
• Long term storage: 100 mM sodium hydrogen carbonate, 0.02% sodium azide, pH 7.5 at 4 °C or 20 mM sodium acetate, 20% ethanol, pH 6.5 at 4 °C
• Shelf life: If properly stored - Three years upon delivery, unopened
• Chemical structure: Carboxy structure

The considerable size of the beads is meant for very viscous cell media and high flow rates. It is meant for protein purification assays of the highest possible purity, exploiting the specificity of an antibody to its antigen. Additionally, we offer Rho1D4-tag affinity resin with a bead diameter of 40 µm for slightly higher protein yield and more mechanical stability of the beads. Alternatively, Rho1D4 MagBeads XL are available if you prefer this affinity chromatography method. Additional support products for Rho1D4-tag purification assays are Rho1D4 peptide, an essential compound of the elution buffer, and our Rho1D4 antibody for later western blot detection of your protein.