- Description:P3 Primary Cell 4D-Nucleofector® X kit S
- Cat. no.:CA10064-502
- No. of Reactions:32
The P3 primary cell 4D-Nucleofector® X kit S is one of our five 4D-Nucleofector® Kits suited for transfecting primary cells in the 20 µl 16-well Nucleocuvette® strip format when working with the 4D-Nucleofector® X Unit.
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
- Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System
This kit has been determined to be optimal for transfecting following cell types:
B cell (human)
CD34+ cell (human)
Chondrocyte (human)
DC (human)
DC (mouse), mature
ES (human), H9
ES (mouse)
Hepatocyte (human)
HMEC (human)
Keratinocyte (human)
Macrophage (human)
Monocyte (human)
Neuron (rat), brain Hi/Cx
NHBE (human)
RPE (human)
T cell (human), stim.
T cell (mouse), unstim.
Applications:
Transfection of low cell numbers in the range of 5×10⁴ to 1×10⁶ cells.
Medium throughput applications.
Optimization of transfection conditions e.g. substrate titrations.
1×0.675 ml P3 primary cell Nucleofector® solution.
1×0.15 ml Supplement 1.
50 µg pmaxGFP® Vector (1 μg/μl in 10 mM Tris pH 8.0).
2×16-well Nucleocuvette® strips (20 μl).