Stem cells possess the unique ability to differentiate into multiple lineages of cells including chondrocytes.

Lonza’s hMSC Chondrogenic Differentiation BulletKit® Medium is a serum containing medium designed to induce chondrogenic differentiation, with the addition of TGF-β3, of human bone marrow derived mesenchymal stem cells into mature, functionally active chondrocytes in as few as 14 days. Differentiation can then be validated by a variety of methods including staining with alcian blue or safranin.

Partial List of Cells Differentiated with hMSC Chondrogenic Differentiation BulletKit® Medium*: Human bone marrow derived stem cells Human umbilical cord blood derived stem cells Human umbilical cord matrix derived stem cells C57BL/6 mice bone marrow derived stem cells human adipose derived stem cells Human hamstring tendon derived stem cells The hMSC Chondrogenic Differentiation BulletKit® Medium, with the addition of TGF-β3 (sold separately) provides a differentiation medium guaranteed to induce chondrogenic differentiation of human bone marrow derived mesenchymal stem cells into mature, functionally active chondrocytes; Each bottle of the hMSC Chondrogenic Differentiation BulletKit® Medium contains enough differentiation medium to fully differentiate at least 2 million human bone marrow derived mesenchymal stem cells [approximately 4 complete 24-well plates] into mature, functionally active chondrocytes.

The hMSC Chondrogenic Differentiation Medium is offered as a BulletKit® Medium (catalog no. PT-3003) which includes both the basal media and the necessary supplements (with the addition of TGF-β3 [sold separately, catalog no. PT-4124]) for chondrogenic differentiation of human bone marrow derived mesenchymal stem cells. The basal medium (Differentiation Basal Medium - Chondrogenic; catalog no. PT-3925) and the necessary supplements (HMSC SQ Kit - Chondro; catalog no. PT-4121 and TGF Beta 3 for HMSC Chondro diff; catalog no. PT-4124) can also be purchased individually.

Lonza guarantees the performance of Clonetics®/Poietics® cells only if appropriate Clonetics®/Poietics® media and reagents are used exclusively and the recommended storage and use protocols are followed.