Trypsin is a clear, colorless-yellow, highly active bovine enzyme preparation, used to remove cells from the culture vessel surface into suspension.It is generally used at a working concentration of 0.025%, prepared in a balanced salt solution, without calcium or magnesium at a pH of 7.6 - 7.8.

After treatment, trypsin activity can be halted by adding a serum media mixture or a trypsin inhibitor (from soybean, for example) in serum-free systems.

Trypsin/EDTA is also a component of Lonza's ReagentPack® Subculture Reagents kit (CC-5034) which includes Trypsin Neutralizing reagent and HEPES buffer.