Poietics® Normal Human Preadipocytes (HprAD) are isolated from subcutaneous or visceral fat from normal (non-diabetic) donors. Preadipocytes from diabetic donors are available as a separate product.

• Test negative for mycoplasma, bacteria, yeast, and fungi
• HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots

Cryopreserved Poietics® Normal Human Preadipocytes (HprAD) are guaranteed to contain ≥1 million or ≥4 million (subcutaneous only) cells per ampoule and test positive for adipogenic differentiation.

Recommended Media and Reagents:
PGM® -2 Preadipocyte Growth Medium-2 BulletKit® Medium (provides both a proliferation medium and a differentiation medium guaranteed to induce differentiation into mature, functionally active adipocytes; Each bottle of the PGM-2® BulletKit® Medium contains enough differentiation medium to fully differentiate 10 million preadipocytes [10 complete 96-well plates] into mature, functionally active adipocytes). The PGM®-2 Medium is offered as a BulletKit® Medium (catalog no. PT-8002) which includes both the basal medium and the necessary supplements for both proliferation and differentiation of preadipocytes. The basal medium (PGM®-2 Basal Medium; catalog no. PT-8202) and the necessary supplements (PGM®-2 SingleQuot® Kit; catalog no. PT-9502) can also be purchased individually.

Lonza guarantees the performance of Clonetics®/Poietics® cells only if appropriate Clonetics®/Poietics® media and reagents are used exclusively and the recommended storage and use protocols are followed.