Nucleofector® Basic Kit for Primary Mammalian Glial Cells
Catalog # CA10064-646
Supplier: LONZA PHARMA - BIOSCIENCE CA
CAS Number:
Specifications
- Description:Basic Nucleofector® kit for primary mammalian glial cells
- Size:25 reactions
- Cat. no.:CA10064-646
- Supplier No.:VPI-1006
Specifications
About this item
In addition to various cell type-specific kits for primary glial cells, we also provide a cell group-specific basic Nucleofector® kit.
- Optimization can be performed within one experiment
- Detailed protocols guide through optimization procedure
- Fine tuning of results is possible
- Transfection efficiency up to 60%
- Viability up to 80%
This kit is suited to determine the optimal transfection conditions for any mammalian glial cell type that lacks a cell type specific optimized protocol for the Nucleofector® 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector® Programs is tested in combination with the basic Nucleofector® solution for glial cells.
Applications:
Kit suited for various glial cells from different mammalian species.
Already tested for rat and mouse astrocytes rat oligodendrocytes.
Delivery: Content:
2.25 ml Nucleofector® solution for primary mammalian glial cells.
0.5 ml supplement 1.
30 µg pmaxGFP® Vector (0.5 μg/μl in 10 mM Tris pH 8.0).
25 aluminum cuvette (100 µl).
25 single use pipettes.
2.25 ml Nucleofector® solution for primary mammalian glial cells.
0.5 ml supplement 1.
30 µg pmaxGFP® Vector (0.5 μg/μl in 10 mM Tris pH 8.0).
25 aluminum cuvette (100 µl).
25 single use pipettes.