The Primary cell optimization Nucleofector® kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an optimized protocol for the 4D-Nucleofector® 96-well unit.

• Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
• Optimal Nucleofection conditions determined on the 4D-Nucleofector® 96-well Unit are transferable to the 4D-Nucleofector® System or the 384-well HT Nucleofector® System

With the unique capability of the 4D-Nucleofector® 96-well Unit to address individual wells of a 96-well Nucleocuvette® Plate with different programs, primary cell optimizations are easily performed within one experiment.

Complete Optimization: Using one 96-well Nucleocuvette® Plate and our pmaxGFP® Vector as positive control, our five Primary Cell Nucleofector® Solutions P1 to P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 32 wells of the second 96-well Nucleocuvette® Plate can be used for optional fine tuning or performing the first experiment with the selected conditions.

Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector® Solutions and Nucleofector® Programs.

Basic Protocols are available for following cell groups:
Endothelial cells (mammalian)
Epithelial cells (mammalian)
Fibroblasts (mammalian)
Glial cells (mammalian)
Neurons (mammalian)
Smooth muscle cells (mammalian)
Stem cells (human)

Applications:
Determination of Nucleofection conditions for primary cell types lacking an optimized protocol for the 4D-Nucleofector® 96-well unit.