Nucleofector® P4 Primary Cell 96-well Kit
CA10064-140
:
- Description:P4 primary cell 96-well Nucleofector® kit
- Cat. no.:CA10064-140
- No. of Reactions:96
- Supplier No.:V4SP-4096
The P4 primary cell 96-well Nucleofector® kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 4D-Nucleofector® 96-well unit.
- High transfection efficiencies for primary cells
- Compatible with liquid handling systems for automated high-throughput electroporation
- Conditions are transferable between 4D-Nucleofector® System, 4D-Nucleofector® 96-well Unit and 384-well Nucleofector® System
This kit has been determined to be optimal for transfecting following cell types:
B cell (mouse)
DC (mouse), immat.
MEF (mouse)
The kit is available in different variants for either 1×96 or 10×96 reactions.
Applications:
Transfection of low cell numbers in the range of 5×10⁴ to 1×10⁶ cells.
High-throughput applications, e.g. s creens of arrayed cDNA, RNAi or CRISPR libraries.
: Content:
2.25 ml P4 primary cell Nucleofector® solution.
0.5 ml Supplement 1.
50 µg pmaxGFP® vector (1 μg/μl in 10 mM Tris pH 8.0).
1×96-well Nucleocuvette® plate (20 μl).
2.25 ml P4 primary cell Nucleofector® solution.
0.5 ml Supplement 1.
50 µg pmaxGFP® vector (1 μg/μl in 10 mM Tris pH 8.0).
1×96-well Nucleocuvette® plate (20 μl).