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Nucleofector® P3 Primary Cell 96-well Kit
Nucleofector® P3 Primary Cell 96-well Kit
Catalog # CA10064-138
CAS Number:  
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Nucleofector® P3 Primary Cell 96-well Kit
Catalog # CA10064-138
Supplier Number:  V4SP-3096
CAS Number:  

Specifications

  • Description:
    P3 primary cell 96-well Nucleofector® kit
  • Cat. no.:
    CA10064-138
  • No. of Reactions:
    96
  • Supplier No.:
    V4SP-3096

Specifications

About this item

The P3 Primary Cell 96-well Nucleofector® kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 4D-Nucleofector® 96-well unit.

  • High transfection efficiencies for primary cells
  • Compatible with liquid handling systems for automated high-throughput electroporation
  • Conditions are transferable between 4D-Nucleofector® System, 4D-Nucleofector® 96-well Unit and 384-well Nucleofector® System

This kit has been determined to be optimal for transfecting following cell types:
B cell (human)
CD34+ cell (human)
Chondrocyte (human)
DC (human)
DC (mouse), mature
ES (human), H9
ES (mouse)
Hepatocyte (human)
HMEC (human)
Keratinocyte (human)
Macrophage (human)
Monocyte (human)
Neuron (rat), brain Hi/Cx
NHBE (human)
RPE (human)
T cell (human), stim.
T cell (mouse), unstim.

Applications:
Transfection of low cell numbers in the range of 5×10⁴ to 1×10⁶ cells.
High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries.

The kit is available in different variants for either 1×96 or 10×96 reactions.

Delivery: Content:
2.25 ml P3 primary cell Nucleofector® solution.
0.5 ml Supplement 1.
50 µg pmaxGFP® vector (1 μg/μl in 10 mM Tris pH 8.0).
1×96-well Nucleocuvette® Plate (20 μl).