Spécifications
- Description:P3 primary cell 96-well Nucleofector® kit
- Cat. no.:CA10064-138
- No. of Reactions:96
- Supplier No.:V4SP-3096
Spécifications
À propos de cet article
The P3 Primary Cell 96-well Nucleofector® kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 4D-Nucleofector® 96-well unit.
- High transfection efficiencies for primary cells
- Compatible with liquid handling systems for automated high-throughput electroporation
- Conditions are transferable between 4D-Nucleofector® System, 4D-Nucleofector® 96-well Unit and 384-well Nucleofector® System
This kit has been determined to be optimal for transfecting following cell types:
B cell (human)
CD34+ cell (human)
Chondrocyte (human)
DC (human)
DC (mouse), mature
ES (human), H9
ES (mouse)
Hepatocyte (human)
HMEC (human)
Keratinocyte (human)
Macrophage (human)
Monocyte (human)
Neuron (rat), brain Hi/Cx
NHBE (human)
RPE (human)
T cell (human), stim.
T cell (mouse), unstim.
Applications:
Transfection of low cell numbers in the range of 5×10⁴ to 1×10⁶ cells.
High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries.
The kit is available in different variants for either 1×96 or 10×96 reactions.
2.25 ml P3 primary cell Nucleofector® solution.
0.5 ml Supplement 1.
50 µg pmaxGFP® vector (1 μg/μl in 10 mM Tris pH 8.0).
1×96-well Nucleocuvette® Plate (20 μl).