The SE Cell Line 96-well Nucleofector® Kit is one of our three kits suited for medium-throughput transfection of cell lines when working with the 4D-Nucleofector® 96-well unit.

• High transfection efficiencies in difficult-to-transfect cell lines
• Compatible with liquid handling systems for automated high-throughput electroporation
• Conditions are transferable between 4D Nucleofector® System, 4D-Nucleofector® 96-well Unit and 384-well Nucleofector® System

This kit has been determined to be optimal for transfecting following cell types:
3T3-L1, diff., 3T3-L1, undiff.
A-10, A7r5
BHK-21, BJ
C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
DU145
GH3
HCT116, HeLa, HeLa S3
IMR-90
Jurkat (DSMZ), Jurkat E6.1
KG-1a, L6
MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MG-63, MRC-5
NCTC clone 929
Panc-1
T/G-HA VSMC, T-47D
U2OS, U-87 MG
WI-38

The kit is available in different variants for either 1×96 or 10×96 reactions.

Applications:
Transfection of low cell numbers in the range of 5×10⁴ to 1×10⁶ cells.
High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries.