Nucleofector® Basic Kit for Primary Mammalian Neurons (SCN)
Catalog # CA10064-652
Supplier: LONZA PHARMA - BIOSCIENCE CA
CAS Number:
Specifications
- Description:Basic Nucleofector® kit for primary mammalian neurons (SCN)
- Cat. no.:CA10064-652
- Supplier No.:VSPI-1003
Specifications
About this item
This is a neural cell specific basic Nucleofector® kit which allows to determine the optimal transfection conditions for any mammalian neuron type. In contrast to our standard basic kit (VPI-1003) this kit is suited for transfection of small cell numbers.
- Efficient transfection of neural or glial cells in small scale (20 µl)
- Optimization can be performed within 1 experiment
- Detailed protocols guiding through the optimization procedure
IMPORTANT NOTE: It only works in combination with an older Nucleofector® Device model, the Nucleofector® IIS Device.
Optimization protocol as well as specific pre-optimized protocols for Mouse DRGs, Rat neurons and Mouse neurons. In these Optimized protocols the best Nucleofection conditions are indicated.
Applications: Transfection of low cell numbers in the range of 2 to 5×10⁴ cells, kit suited for various neuron types from different mammalian species.
Delivery: Content: 0.45 ml basic neuron SCN Nucleofector® solution, 0.1 ml supplement 1, 30 µg pmaxGFP® vector (0.5 μg/μl in 10 mM Tris pH 8.0), 25 aluminum SCN cuvettes (20 µl), 25 single use pipettes.